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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Thermococcus celer ： ウィキペディア英語版 Thermococcus celer ''Thermococcus celer'' is a Gram-negative, spherical shaped archaeon of the genus ''Thermococcus''.〔Achenbach-Richter, L., R. Gupta, W. Zillig, C. R. Woese. 1988. Rooting the Archaebacterial Tree: The Pivitol Role of Thermococcus celer in Archaebacterial Evolution. Syst. Appl. Microbial. 10:231-240. Print.〕 The discovery of ''Thermococcus celer'' played an important role in re-rooting the tree of life when it was discovered that ''T. celer'' was more closely related to methanogenic Archaea than to other phenotypically similar thermophilic species 〔 ''T. celer'' was the first archaeon discovered to house a circularized genome.〔Noll, K M. 1989. “Chromosome Map of the Thermophilic Archaebacterium Thermococcus celer.” Journal of Bacteriology 171.12: 6720–6725. Print.〕 Several type strains of ''T. celer'' have been identified: Vu13, ATCC 35543, and DSM 2476.〔 ==Isolation== ''T. celer'' was discovered by Dr. Wolfram Zillig in 1983.〔 The organism was isolated on the beaches of Vulcano, Italy from a sulfur rich shallow volcanic crater.〔 Original samples were isolated from the depths of the marine holes and inoculated into 10 mL anaerobic tubes.〔Zillig, W., K. O. Stetter, W. Schafer, D. Janekovic, S. Wunderl, I. Holz, and P. Palm. "Thermoproteales: Novel Order of Archaebacteria." Zentralblatt Fur Bakteriologie Mikrobiologie Und Hygiene 2 (1981): 205-27. Print.〕 The tubes contained 100 mg of elemental sulfur as well as a solution of 95% N2 and 5% H2S.〔 The pH was subsequently adjusted to a range of 5-6 through the addition of CaCO3.〔 To ensure that no oxygen had permeated the sample, researchers utilized the oxygen indicator resazurin.〔 Growth was achieved by enrichment with Brock’s ''Sulfolobus'' medium, which contains elemental sulfur and yeast, both of which are required by ''T. celer'' for optimal growth.〔 Following enrichment, the samples were plated onto polyacrylamide gel and then incubated at 85° Celsius in an anaerobic environment.〔 Once colony growth had been observed, the cells were subjected to centrifugation prior to purification in a TA buffer solution (0.05 mol/1 Tris HCL, 0.022 mol/1 NH4Cl, 0.01 mol/1 β-mercaptoethanol).〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Thermococcus celer」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.